Measurement of microbial biomass N:C by chloroform fumigation-incubation

Measurement of microbial biomass N:C by chloroform fumigation-incubation

Harris, D., Voroney, R. P. and Paul, E. A. 1997. Measurement of microbial biomass N:C by chloroform fumigation-incubation. Can. J. Soil Sci. 77: 507–514. We present a calculation for soil microbial biomass N:C ratio determined from a 10-d incubation following chloroform fumigation. The calculation is based on a mathematical model of the N content of the preand post-fumigation soil microbial bio...

Uncertainties in determining microbial biomass C using the chloroform fumigation–extraction method

Soil Microbial Biomass Determination

Basic Idea: The quantity of microbial biomass in a given soil sample is inherently difficult to measure. To some degree, any measurement of microbial biomass is relative – different methodologies or variations in methodologies will yield microbial biomass estimates that are not directly comparable. For this reason, if you want to measure microbial biomass – choose a method and stick with it exa...

Tillage Effects on Intracellular and Extracellular Soil Urease Activities Determined by an Improved Chloroform Fumigation Method

SoilScience • Volume 175, Number 11, November 2010 Urease is one of the most thoroughly studied soil enzymes because it greatly influences the fate and performance of a widely used fertilizer (urea) (Bremner and Mulvaney, 1978; Ma et al., 1999; Tate, 2002). Urea was reported to be taken up by microorganisms, such as Corynebacterium glutamicum and Paxillus involutus (Jahns and Kaltwasser, 1989; ...

Responses of soil microeukaryotic communities to short-term fumigation-incubation revealed by MiSeq amplicon sequencing

In soil microbiology, there is a "paradox" of soil organic carbon (SOC) mineralization, which is that even though chloroform fumigation destroys majority of the soil microbial biomass, SOC mineralization continues at the same rate as in the non-fumigated soil during the incubation period. Soil microeukaryotes as important SOC decomposers, however, their community-level responses to chloroform f...